Postdoc in Single Cell RNA Sequencing Bioinformatics

Type 2 diabetes is a devastating disease and the mechanisms behind are not fully understood. We use different single cell RNA sequencing protocols in combination with comprehensive experimental validation as part of our long-standing effort to understand Type 2 diabetes-induced alterations in the pancreatic islets, as well as in intestinal enteroendocrine cells. Our development of novel calculation tools (based on disease-related coordination of gene expression) has led to a major leap forward for identifying cell type specific disease mechanisms in islet beta cells. Identified genes have successfully been functionally validated (in human tissue, cell lines and in vivo in mice) and new disease mechanisms have been established. This study was recently published in Nat Comm (https://pubmed.ncbi.nlm.nih.gov/41145477/). We now focus our efforts on experimental and clinical studies of the enteroendocrine cells in the small intestine. Clinical samples and mouse models have been analyzed using SPLiT-Seq and 10X. The aim is to understand cell type specific impact of Type 2 diabetes on each of the eight hormone producing cell populations, as well as potential coordinated changes in each of the five islet cell types (in mouse models). Responsibilities The project needs a creative bioinformatician who is capable of developing new ways of assessing single cell RNA sequencing data as well as to refine existing calculation pipelines. Ideally, we would need a person possessing these skills combined with experience in the wet lab parts of single cell RNAseq or other RNA work. Qualifications

• To be eligible for a post-doc scholarship at Lund University the recipient must hold a PhD degree within a relevant field. The PhD degree must not be from Lund University.
• The PhD degree must not be older than three years.
• The applicant must not have been employed at Lund University in the past two years.
• Proven skills in single-cell bioinformatics
• Experience in molecular biology and cell biology
• Excellence in English language